Custom Additives

Need a custom additive not provided by Nuclera? Nuclera also offers guidance for you to make your own additives to test on the eProtein Discovery™ system. See our Custom Additives Guide for more information. The development of membrane protein on the eProtein Discovery™ led Nuclera to the identification of suitable nanodiscs and detergent to be used on the platform. A guide to the selection of nanodiscs can be found below.

Nanodiscs Selection guide

For membrane proteins, one stabilizing custom additive (e.g., a nanodisc, which mimics lipid bilayers to support proper folding and function) should be selected, along with either an additional additive from Nuclera's standard additive panel or another compatible custom additive.

If the membrane protein is required for immunization, select the appropriate species. If not, Human should be used. Alternative nanodiscs not listed can also be screened, but please consult our Chemical Compatibility guide or contact our technical support team at techsupport@nuclera.com for guidance.

Select Membrane Scaffold Protein

Select tags

Select between optional tags. His-tagged or untagged membrane scaffold proteins for ease of purification if assembling own nanodiscs. Preassembled nanodiscs with biotin labeled phospholipids for SPR.

Select phospholipids

For the selection of the extra additive follow the [diagram](Link to general information section) at the top of this page

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Email: techsupport@nuclera.com

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